LncRNA CCAT1 promotes cell proliferation and differentiation via negative modulation of miRNA-218 in human DPSCs.

Abstract

To investigate the expression of Long non-coding RNA (LncRNA) CCAT1 and potential functions in promoting cell proliferation and differentiation, via miRNA-218 in human adult Dental Pulp Stem Cells (DPSCs). CCAT1 expressions in Periodontal Ligament Cells (PDLCs), DPSCs, differentiated main population (MP) cells and stem-cell-enriched Side Population (SP) cells in DPSCs were detected by qRT-PCR. MTT assay and ELISA assay were performed to evaluate the DPSCs cell proliferation and differentiation. The correlation between miR-218 and CCAT1 was detected by statistical analysis. The bioinformatics and luciferase assay were performed to explore the interaction and binding site of CCAT1 and miR-218. Results showed the CCAT1 expression was up-regulated in DPSCs cells. And the expression level in MP cell was higher than SP cell. MTT assay and showed overexpression CCAT1 significantly increased cell proliferation of DPSCs. ELISA assay showed the expressions of collagen I, Osteopontin (OPN) and Osteocalcin (OCN) were significantly increased in DPSCs compared with control (p<0.05). The bioinformatics and luciferase assay showed that the CCAT1 directly interacted with miR-218. In addition, miR-218 expression was negatively correlated with CCAT1 expression in DPSCs CONCLUSIONS: For the first time, we found that lncRNA-CCAT1 was upregulated in DPSCs, which could promote cell proliferation and differentiation by repressing the expression of miR-218.