Abstract
BackgroundRecurrent aphthous stomatitis (RAS) is a common oral disease with unknown molecular pathogenesis. Our preliminary microarray analysis revealed the altered expression of lncRNA Cancer Susceptibility Gene 2 (CASC2) in RAS. We therefore analyzed the role of CASC2 in RAS.MethodsIn this study, plasma samples were obtained from RAS patients and healthy participants. Plasma levels of CASC2 were measured by RT-qPCR. Plasma levels of IL-6 and IL-18 were measured by enzyme-linked immunosorbent assay (ELISA). A follow-up study was performed to analyze the role of CASC2 in the recurrence of RAS.ResultsIn the present study, we found that lncRNA Cancer Susceptibility Gene 2 (CASC2), as well as pro-inflammatory factors interleukin 6 (IL-6) and interleukin 18 (IL-18), were upregulated in plasma of RAS patients compared with healthy participants. Plasma levels of lncRNA CASC2 were positively correlated with plasma levels of IL-6 and IL-18 in RAS patients but not in healthy participants. Compared with pre-treatment levels, plasma levels of lncRNA CASC2, IL-6 and IL-18 were reduced after recovery. A follow-up study showed that patients with high levels of lncRNA CASC2 had a significantly higher recurrence rate.ConclusionLncRNA CASC 2 is upregulated in RAS and predicts the recurrence.
Highlights
Recurrent aphthous stomatitis (RAS) is a common oral disease with unknown molecular pathogenesis
Cancer Susceptibility Gene 2 (CASC2), interleukin 6 (IL-6), and interleukin 18 (IL-18) were upregulated in RAS patients The differential expression of CASC2, IL-6, and IL-18 was analyzed by performing qPCR and enzyme-linked immunosorbent assay (ELISA) experiments
It was observed that compared to the Control group, plasma levels of CASC2 (Fig. 1a), IL-6 (Fig. 1b) and IL-18 (Fig. 1c) were significantly higher in the RAS group (p < 0.001)
Summary
Recurrent aphthous stomatitis (RAS) is a common oral disease with unknown molecular pathogenesis. Recurrent aphthous stomatitis (RAS) is a commonly diagnosed and the recurrent inflammatory process in which ovoid or round painful ulcers of the oral mucosa recur [1, 2]. It has been well-established that food sensitivity, trauma, systemic conditions, nutritional deficiencies, immunological disorders and infections of Helicobacter pylori are closely correlated with the occurrence and progression of RAS [3]. More than 50% of patients with RAS will experience recurrence within 3 months after treatment and the long-term non-recurrence is rare. Long noncoding RNAs (lncRNAs, > 200 nt) have no ability in
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