Abstract
sBackgroundsLncRNA Brain Cytoplasmic RNA 1 (BCYRN1) has been certified to modulate cancer cells growth and aggressiveness in several tumors. However, research about function of BCYRN1 in hepatocellular carcinoma (HCC) is limited. Therefore, our research intends to explore the function of BCYRN1 in HCC.MethodsHepG2 and BEL-7402 cell lines were employed for later function experiments. Differently expression levels of BCYRN1, miR-490-3p, and POU class 3 homeobox 2 (POU3F2) were determined on the base of TCGA dataset including 375 HCC patients and 50 normal. 370 cases of patients, which have fairly complete clinical data, were utilized for survival analysis of BCYRN1, miR-490-3p, or POU3F2 by Kaplan–Meier method. Relative expression pattern of BCYRN1 was examined by quantitative real time polymerase chain reaction (qRT-PCR), and relative expression level of POU3F2 was assessed by qRT-PCR and western blot. Cell biological behaviors were analyzed by cell counting kit-8, cloning formation, and transwell assays. Bioinformatics software and dual luciferase assay were applied to predict and confirm the targeted relationship between BCYRN1 and miR-490-3p, as well as miR-490-3p and POU3F2. Further associations among BCYRN1, miR-490-3p, and POU3F2 were analyzed by rescue assays.ResultsOur results exhibited that BCYRN1 was over expressed in HCC samples, which was connected with unfavorable prognosis in HCC patients. In addition, a series of experiments exhibited that overexpression of BCYRN1 significantly expedited HCC cells growth, clone formation, and movement abilities, and vice versa. Moreover, targeted relationships between BCYRN1 and miR-490-3p, as well as miR-490-3p and POU3F2 were affirmed by dual luciferase assay. Furthermore, POU3F2 expression was negatively connected with the expression of miR-490-3p and positively associated with BCYRN1 expression. Whilst, either overexpression of miR-490-3p or knockdown of POU3F2 could remarkably inhibit the increasing trends of proliferation, clone formation, invasion, and migration abilities induced by BCYRN1 in HCC cells.ConclusionsBCYRN1, served as a competing endogenous RNA, up-regulated the expression of POU3F2 to promote the development of HCC through sponging miR-490-3p, supplying novel molecular targets and underlying prognostic biomarkers for HCC therapy.
Highlights
Hepatocellular carcinoma (HCC) is the most frequent malignant liver tumor type in the world, which is an important problem affecting human health [1]
Our results exhibited that Brain Cytoplasmic RNA 1 (BCYRN1) was over expressed in HCC samples, which was connected with unfavorable prognosis in HCC patients
Either overexpression of miR-490-3p or knockdown of POU class 3 homeobox 2 (POU3F2) could remarkably inhibit the increasing trends of proliferation, clone formation, invasion, and migration abilities induced by BCYRN1 in HCC cells
Summary
Hepatocellular carcinoma (HCC) is the most frequent malignant liver tumor type in the world, which is an important problem affecting human health [1]. It is urgent to reveal the underlying molecular mechanisms of HCC and search for the target molecules for early diagnosis and prognosis. Among numerous lncRNAs, BCYRN1 has been reported to be a critical molecule to regulate cancer cells survival and proliferation [6]. Study from Gu et al has suggested that BCYRN1 regulated proliferation of colorectal cancer cells through up-regulating NPR3 expression [8]. Recent research indicated that BCYRN1 accelerated the proliferation and metastasis of cervical cancer through regulating miR-138 in vitro and in vivo [9]. Research from Ren et al found that overexpression of BCYRN1 facilitated tumor progression and up-regulated EpCAM expression in gastric carcinoma [10]. Our research mainly focused on the regulatory network of BCYRN1 in HCC
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