Abstract
Objective Colorectal cancer (CRC) is the most common cancer. But, the molecular mechanisms of CRC progression are not fully understood. This study was conducted to explore how the long noncoding RNA actin filament-associated protein 1-antisense RNA1 (lncRNA AFAP1-AS1) participates in CRC progression through the regulation of microRNA-195-5p (miR-195-5p) and wingless-type inducible signaling pathway protein-1 (WISP1). Methods The expressions of AFAP1-AS1, miR-195-5p, and WISP1 were detected by RT-qPCR or western blot. A dual-luciferase assay confirmed the target relationship of AFAP1-AS1, miR-195-5p, and WISP1. Colony formation, wound-healing, and Transwell assays were used to detect the growth, migration, and invasion abilities of cells, respectively. Results AFAP1-AS1 and WISP1 expressions were notably increased, and miR-195-5p expression was markedly reduced in CRC. The dual-luciferase assay verified that AFAP1-AS1 could bind to miR-195-5p. AFAP1-AS1 knockdown could inhibit the malignant behavior of CRC cells. miR-195-5p could target and regulate WISP1 expression. Overexpression of WISP1 or miR-195-5p inhibition reversed the inhibition effect of AFAP1-AS1 knockdown on the biological activity of CRC cells. Conclusions AFAP1-AS1 knockdown may inhibit the proliferation, migration, and invasion of CRC cells through the miR-195-5p/WISP1 axis.
Highlights
Colorectal cancer (CRC) is a major cause of morbidity and mortality throughout the world
With the in-depth research on lncRNAs, more and more lncRNAs were revealed to have a vital role in CRC pathogenesis and progression. eir biological functions include the regulation of proliferation, apoptosis, tumor resistance, and metastasis of CRC, which have great value in clinical diagnosis and prognosis judgment [6]
To verify the role of AFAP1-AS1 in CRC, siAFAP1-AS1 was transfected into SW480 cells, and AFAP1AS1 expression was assessed by RT-qPCR
Summary
Colorectal cancer (CRC) is a major cause of morbidity and mortality throughout the world. E invasion and metastasis of malignant tumors is often the main cause of treatment failure, and 40% to 50% of patients with CRC die from distant metastasis [3]. Erefore, in-depth study on the etiology and pathogenesis of CRC is of great significance for clinical diagnosis and treatment. Eir biological functions include the regulation of proliferation, apoptosis, tumor resistance, and metastasis of CRC, which have great value in clinical diagnosis and prognosis judgment [6]. Recent studies have found that AFAP1AS1 is highly expressed in many tumors and involved in the regulation of tumorigenesis and development [8]. Journal of Oncology studies on AFAP1-AS1 have shown that AFAP1-AS1 is upregulated in CRC, suggesting that the high expression of AFAP1-AS1 involves CRC occurrence and development [9,10,11], but the specific mechanism of its involvement in tumor-related processes remains unclear
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