Abstract
Nowadays, research on CSCs is still in an initial stage, and there are few studies reporting the successful isolation and identification of CSCs. In the present study, we attempted to isolate CSCs through cultivating the cell line MKN45 in defined serum‐free medium and study the expression of stem cell markers or related proteins (Oct3/4, Sox2, Nanog and CD44) in CSCs. Moreover, immunofluorescence staining was performed to validate the stem cell markers of spheroid body‐forming cells. Further experiments were used to evaluate the SPOP expression in tumorsphere cells. In addition, ADAMTS9‐AS2 is a lncRNA that contributes to the genesis and development of many cancers, including gastric cancer (GC). We found ADAMTS9‐AS2 functioned as an anti‐oncogene and positively correlated with the expression of SPOP in GC tissues by combining bioinformatics analyses. Furthermore, we reported that ADAMTS9‐AS2 regulated the expression of SPOP in GC cells and tumorsphere cells to inhibit GC progression. Together, our results demonstrated that SPOP and ADAMTS9‐AS2 can be potential targets for GC treatment.
Highlights
Gastric cancer (GC) is one of the most common groups of malignancies in the world
We further studied the influences of Speckle-type POZ protein (SPOP) and ADAMTS9-AS2 on proliferation, apoptosis, and the cycle of GC cells and spheroid formation of tumorsphere cells, in an attempt to explore the potential effects of ADAMTS9-AS2 and SPOP in the initiation and progression of GC
The above results demonstrated that ADAMTS9-AS2 suppressed the proliferative ability of GC cells and spheroid formation of tumorsphere cells was mediated by the down-regulation of SPOP in vitro
Summary
Gastric cancer (GC) is one of the most common groups of malignancies in the world. There are one million new cases worldwide each year, mainly in developing countries, especially in China. | 4831 there is a small part of cells in the tumour tissue, and they can possess self-renewal and multi-directional differentiation potential and strongly associated with the malignant degree of the tumour, drug resistance and metastasis, which plays a crucial role in the development and progression of tumour.[3] the American Association for Cancer Research identified these cells as self-renewal, relatively static, multidrug-resistant and multipotent cells in cancer tissue, as well as cells with stronger tumorigenicity and in vivo metastasis, as compared with other cancer cell lines These cells were deemed cancer stem cells (CSCs).[3] Tumour stem cells can proliferate migrate and produce tumours in an appropriate microenvironment. The bands were scanned using an Odyssey infrared imaging system (LI-COR, Lincoln) and quantified
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