LMD-assisted single cell DNA typing of forensic biological evidence: Issues of the cell type and sample condition

Abstract

The practice of DNA typing of single cells in forensic biological evidence may have peculiarities. For instance, some laser microdissected (LMD) single cell preparations fail to give any PCR products while there is no such a problem with macropreparations from the same sample. As an answer, this study shows that not all the cells individually retrieved from the forensic sample are suitable for analysis. Hence, when going from the bulk to single cell preparations, a bottleneck occurs, which reduces chances for successful DNA typing. For a useful single cell analyses in forensics, single cell DNA profile recovery dependency on the cell type and sample condition must be considered.