Abstract

This report summarizes research related to Lawrence Livermore National Laboratory's (LLNL) Experimental Test Site, Site 300 (S300), located within Alameda and San Joaquin Counties (Figure 1) and conducted under the 10(a)(1)(A) (Recovery) permit TE-053672-2. The U.S. Department of Energy/National Nuclear Security Administration (NNSA) holds this property in ownership. The 2006 recovery research at S300 involved fieldwork associated with two species: the California whipsnake (Masticophis lateralis) (MALA) and the California red-legged frog (Rana aurora draytonii) (CRLF). A research project (Biological Opinion 1-1-02-F-0064) investigating the direct effects (fatality/harm) and indirect impacts (habitat alteration) of prescribed burning of coastal sage scrub on MALA was permitted by the U.S. Fish and Wildlife Service (Service), Sacramento Office, in the Spring of 2002. Several different public resource management agencies were approved by the Service to implement recovery-oriented (fuels treatment) studies associated with this research plan. LLNL's proposed seven-year study was initiated in 2002 and is anticipated to conclude in 2008. Results reflected in this report apply to information gathered during the first five years of the project; because of the low MALA sample size collected onsite and the unforeseen wildland fire that burned both of the study sites in 2005, long term conservation-related measures for MALAmore » are not yet evident from study analyses. The CRLF research (Biological Opinion 1-1-02-F-0062) involved translocating individuals from two wetland locations that had received artificial (potable) water discharges for the last 15-20 years. CRLF's that occupied the wetland sites were moved to an enhanced wetland area further downstream (engineered pools in a pre-existing perennial drainage) in an area called ''Mid-Elk Ravine''. The aboveground, potable water flows were terminated once all CRLF translocations had been completed (Summer of 2006).« less

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