Abstract
Abstract The C1q domain containing (C1qDC) proteins are defined as a family of all proteins containing the globular C1q (gC1q) domain in the C-terminus. They participate in a series of immune responses depending on versatile gC1q domain which can bind a variety of self and non-self ligands. In this study, a novel C1qDC protein was identified from Lampetra japonica (designated as LjC1qDC-1). Its full-length cDNA was of 1662 bp with an open reading frame (ORF) of 711 bp encoding a polypeptide of 236 amino acids. The deduced protein contained a signal peptide, a collagen-like sequence with Gly-Xaa-Yaa repeats, and a typical gC1q domain. Real-time PCR analysis showed that the transcript of LjC1qDC-1 gene was distributed in various immune associated tissues of lamprey. This observation was further confirmed by FACS and confocal laser scanning microscope. In addition, LjC1qDC-1 interaction with specific VLR was analysised by co-immunoprecipitation and western blot. All these results indicated that LjC1qDC-1 gene identified may play an important role in the immune responses of lamprey, which provided a clue to understand the universal function of C1qDC proteins in different species.
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