Abstract
Phospholipid hydroperoxide glutathione peroxidase (GPx4/PHGPx) is a most abundant selenoprotein in the brain and GPx4 null mice die in utero at midgestation or knockdown of GPx4 during mammalian embryogenesis disturbs brain development. The current experiments were designed to investigate, by semiquantitative RT-PCR, the cerebral function of GPx4 of the lizards collected in both polluted (Sarno River, Italy) and unpolluted (Lago Matese, Italy) areas in order to provide useful molecular information on this widely distributed seleno-enzyme in adult as well through the development of the lizard, Podarcis sicula. Our research demonstrates the lizard GPx4 hormonal control. Treatments with HCG increases immunoreactivity while the anti-estrogen ICI 182–780 induces its reduction. The partial region of the cloned cDNA is of 340 bp; conserved protein domains reports its membership to selenoprotein and analysis amino acid residues the value as an antioxidant. The semiquantitative expression of GPx 4 of adult lizard brain in polluted station resulted generally very high and let sustain its importance as lizard neuroendocrine disruptor alarm.
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