Abstract
Abstract Recent studies strongly suggest that the liver plays an im portant immunoregulatory role. Ev idence of its role in general immune responsiveness originates from ob servation that, in recipients of liver grafts, the survival of other allo-grafts is significantly prolonged. The question arises as to which blood lymphocyte subsets, most likely to be responsible for this phenomenon, marginate in liver sinusoids. To study this problem, a liver ex vivo perfusion model was designed for rats. In situ W/WAG livers were wa shed clear of sinusoidal marginating cells prior to and after 1 h perfusion with syngeneic blood. The number of blood cells retained in liver sinu soids, their phenotypes, the respon siveness to mitogen (PHA, 90 μg/ ml) and cytotoxicity against YAC-1 tumour cells were examined. Our studies showed that rat liver retains in the sinusoids a population of blood cells, enriched in NK, CD8′ and MHC class II+ cells, displaying a high cytotoxic activity and low re sponsiveness to mitogen stimula tion, with a capacity of about 106 cells/g of tissue.
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