Liver histological structure of rats (Rattus norvegicus) in the lactation period after supplemented with organic quail eggs

Abstract

Lactation period requires more nutrients and energy to meet the needs of lactating rats and pups. Increased energy and nutrient requirements during lactation have an effect on increasing metabolic rate as well. Lactation energy deficiency may affect the histology of the liver. This study was conducted to examine and analyze the effect organic quail eggs supplementation on hepatocytes diameter, hepatic lobules diameter, liver weight, and body weight in the lactating test animal. This study used 20 females Wistar rats consisted of 5 treatments with 4 replications. The treatment of this study consisted of T0: control rat; T1: rat supplemented with commercial quail eggs; T2: rat supplemented with standard organic quail eggs; T3: rat supplemented with organic quail eggs contained cassava leaves, mackerel, and turmeric powder; and T4: rat supplemented with organic quail eggs contained cassava leaves, seaweed, and turmeric powder. This research used Completely Randomized Design. The variables measured in this study were hepatocytes diameter, hepatic lobules diameter, liver weight, and body weight in the test animal. The obtained data were analyzed using Analysis of Variance (ANOVA) at 5% significance level and continued with Duncan test with 5% significance level if there is a significant difference. The result of data analysis showed that supplementation of organic quail eggs give the significant difference in hepatocytes diameter and hepatic lobules diameter, but had no significant difference in liver weight and body weight so that it can be concluded that quail eggs supplementation in the lactation period affects rats’ liver histological structure.