Abstract
Human epidermal growth factor (EGF) and rat recombinant transforming growth factor alpha (TGFa) stimulated in a dose dependent manner the proliferation of rat liver fat storing cells (FSC) grown as primary culture in Dulbecco's modification of Eagles medium (DMEM) with 0.5% fetal calf serum (FCS). Cell proliferation was measured by DNA-content, [ 3H]thymidine- and bromodeoxyuridine-incorporation, respectively. Half maximal stimulation of [ 3H]thymidine incorporation was seen with 8ng/ml EGF and 7 ng/ml TGFa, respectively. In a radioreceptor assay using [ 125I]EGF as ligand TFGa binds with the same affinity as EGF to EGF-cell surface receptors on FSC. Eight ng/ml TGFa was required to produce half-maximal inhibition of [ 125I]EGF binding. If transforming growth factor beta (TGFβ) was added simultaneously with EGF or TGFa, FSC proliferation was inhibited strongly.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Biochemical and Biophysical Research Communications
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
