Liver cancer antiproliferative activity of a new nor-cucurbitacin from Mareya micrantha Müll. Arg.

Abstract

Mareya micrantha Müll. Arg. (Euphorbiaceae) is a plant used in the Ivorian traditional medicinal system for various medical properties such as laxative, oxytocic, intestinal infectious diseases, malaria, etc. Six cucurbitacin derivatives (tetracyclic triterpenoids) are isolated from the leaves, stem barks or root barks of M. micrantha. Among these compounds, 29-nor-2β,15α,20β-trihydroxy-16α-acetyl-3,1,22-trioxo-cucurbita-4,23-diene (1) is a new nor-cucurbitacin isolated from the leaves; 29-nor-1,2,3,4,5,10-dehydro-3,15α,20β-trihydroxy-16α-acetyl-11,22-dioxo-cucurbita-23-ene 2-O-β-D-glucopyranoside (2) and 29-nor-2β,15α,20β-trihydroxy-16α-acetyl-3,11,22 trioxo-cucurbita-4,23-diene 2-O-β-D glucopyranoside (3) are new nor-cucurbitacins recently discovered by us in leaves and isolated again for this study while dihydro-epi-isocucurbitacin D (4), tetrahydro-cucurbitacin I (5) and cucurbitacin L (6) are known cucurbitacins but newly isolated from the stem barks and the root barks of M. micrantha. Their chemical structures are established according to spectral data (UV, IR, MS and 1H, 13C NMR). Their antiproliferative activity is explored in vitro on the chemo-resistant human hepatocarcinoma cell line Hep3B. Compound 1 showed a strong and selective antiproliferative activity against this cancer cell line (IC50 value of 0.12 ± 0.05 μM) when compared to normal hepatocytes HepaRG.