Liver and Its Lymph Region at Benzo[a]pyrene Effects in an Experiment

S.V Michurina,S.I Kolesnikov,I.Yu Ishchenko,V.I Konenkov,Yu.I Borodin

doi:10.15690/vramn.v70i2.1319

Abstract

Benzo[a]pyrene (BaP) is the widespread environmental toxicant with carcinogenic activity. BaP undergoes metabolic activation in the liver microsomal monooxygenase system, and its regional lymph nodes act as peripheral filters, purifying lymph formed in the liver. It remains an open question about the significance of the integral liver and lymphatic system work in supporting processes of adaptation and resistance to the BaP effects. The purpose of our investigation was to study structural and metabolic changes in the liver and its lymph region in males Wistar rats weighing 180-220g. Animals of the experimental group (n =20) daily for 3 days was performed BaP injections: intraperitoneal with 2 mg per 100g of body weight in 0.2-0.3 ml of olive oil. Rats in the control group (n =20) received in the same mode of injection of olive oil. The light and electron microscopy morphometric study of the liver and its regional lymph nodes morphometric analysis were performed. The intensity of lipid peroxidation in the liver was measured by the number of diene conjugates (DC), ketotriene conjugates (KC) and malondialdehyde (MDA). Simultaneous increase of the hepatic sinusoids relative area and the specific area of the regional lymph nodes sinus system under the BaP effect was found. At ultrastructural level dilatation of the Disse spaces, filling of cell detritus and collagen fibers bundles of these spaces were noted. Damage of nuclear apparatus, balloon transformation of granular endoplasmic reticulum profiles were found in hepatocytes, condensation of the mitochondrial matrix was observed. The relative squares of B-dependent zones increased and T-dependentparacortical zone reduced in the regional lymph nodes. The increase in the content of KC and MDA in liver was identified. benzo[a]pyrene causes interrelated cascade of reactions in the liver and in its lymphatic region: a disturbance of the blood-lymph barrier morphological organization, and so obstruction of the lymphatic drainagefrom the organ. These promotes development of tissue hypoxia and apoptosis, protein synthesizing and energy cell apparatus disruptions, formation of lymphoid tissue temporary infiltrates in the liver.

Highlights

Benzo[a]pyrene (BaP) is the widespread environmental toxicant with carcinogenic activity
BaP undergoes metabolic activation in the liver microsomal monooxygenase system, and its regional lymph nodes act as peripheral filters, purifying lymph formed in the liver
It remains an open question about the significance of the integral liver and lymphatic system work in supporting processes of adaptation and resistance to the BaP effects

Summary

Научные сообщения

Бенз[a]пирен (БаП) — широко распространенный в окружающей среде токсикант, обладающий канцерогенной активностью. Остается открытым вопрос о значимости интегральной работы печени и лимфатической системы в обеспечении процессов адаптации и резистентности к воздействиям БаП. Методы: животным опытной группы (n =20) ежедневно в течение 3 сут проводили инъекции БаП внутрибрюшинно по 2 мг на 100 г веса тела в 0,2–0,3 мл оливкового масла. Выполняли морфометрическое исследование светооптических препаратов и электроннограмм печени, морфометрический анализ ее регионарных лимфатических узлов. Результаты: при воздействии БаП происходит синхронное увеличение относительной площади печеночных синусоидов и удельной площади синусной системы регионарных лимфатических узлов. Заключение: воздействие бенз[a] пирена приводит к развитию взаимосвязанного каскада реакций в печени и ее лимфатическом регионе: нарушению организации гемато лимфатического барьера, изменению корней лимфатической системы в печени, затруднению оттока лимфы от органа. Ключевые слова: бенз[a]пирен, печень, пространство Диссе, лимфатические узлы, кетотриеновые конъюгаты, малоновый диальдегид.

Background

Выражение признательности