Abstract
By using the immunological method of Hales and Randle for the determination of insulin, we have found positive values indicative of the presence of insulin, in human, rabbit, rat, dog, swine, cat, sheep, chicken and ox bile. Homologous and heterologous insulin added to the bile specimens were recovered up to 100 per cent. Determination of the insulin in various bile dilutions shows a direct ratio between the degree of dilution and the estimated concentration. Dialysis of the bile in Visking tubes preserves 82 per cent of its insulin activity determined with the anti-insulin antibody. Bile insulin is destroyed by incubation with cystein at alkaline pH. The insulin concentration in the bile flow is increased after intravenous injection of glucose, used as a load test. There is an increased concentration of insulin in the rabbit bile depending on the time after injection and the amount of homologous insulin infused into the portal vein.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
