Abstract
Gene regulation is intrinsically dynamic: from the microscopic molecular events underlying transcriptional bursting, to genes cross-regulating each other in the context of a genetic network during cellular fate specification. However, knowledge about the transcriptional dynamics of individual genes and the coordinated dynamics of multiple interacting genes in their endogenous context is almost completely missing. We have developed an optimized two-photon microscope to measure real-time gene activity in early fly embryos.
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