Abstract
Growth and death are both fundamental macroscopic properties for all living matters, and thus cell division and mortality rates are good parameters for characterizing cellular physiology in a given environment. While population growth rates in various conditions have been reported in literature, death rate is rarely measured, especially in favorable culture conditions where cells grow exponentially. In our recent study (Nakaoka and Wakamoto, 2017), we developed a microfluidics-based platform to track multiple single cell lineages until death. The system enabled us to monitor both cell growth and death in controlled steady environments, and we confirmed the absence of replicative aging in fission yeast old-pole cell lineages by showing remarkable constancy both in cell division and mortality rates. Furthermore, we revealed a growth-death trade-off relation in non-stressed conditions. The phenomenological law that constrains macroscopic physiological parameters could provide a new quantitative insight into possible balanced-growth states in various environments.
Highlights
Fission yeast old-pole cell lineages do not age under favorable conditions Replicative senescence or aging in unicellular organisms is defined as progressive decline in proliferative activity and increase in mortality rate as cells undergo divisions
The system enabled us to monitor both cell growth and death in controlled steady environments, and we confirmed the absence of replicative aging in fission yeast old-pole cell lineages by showing remarkable constancy both in cell division and mortality rates
The mother cell undergoes replicative aging, and molecular markers correlated with the aging phenotypes, such as ERCs, high cytosolic and vacuolar pH, carbonylated proteins, and protein aggregation, have been postulated
Summary
Fission yeast old-pole cell lineages do not age under favorable conditions Replicative senescence or aging in unicellular organisms is defined as progressive decline in proliferative activity and increase in mortality rate as cells undergo divisions. Growth and death are both fundamental macroscopic properties for all living matters, and cell division and mortality rates are good parameters for characterizing cellular physiology in a given environment.
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
