Abstract
Brillouin microscopy is a noncontact all-optical method for biomechanical imaging. Whilst spontaneous Brillouin scattering microscopy has been widely used in biology, stimulated Brillouin scattering (SBS) microscopy has been mainly used for the examination of materials. Recently, SBS microscopy has been demonstrated useful for the mechanical imaging of live Caenorhabditis elegans nematodes. Here, we will present SBS microscopy of live NIH/3T3 cells, with no apparent photodamage for ~260mW optical power on the samples. We will discuss the importance of multicomponent spectral analysis of the SBS spectra acquired along with the high spectral resolution of the SBS microscope using information theoretic measures.
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