Abstract
Calcium (Ca2+) is an intracellular messenger that plays an essential role in a variety of cellular processes ranging from early embryonic events to muscle contraction and neuron excitability. Measurement of cytosolic, endoplasmic reticulum (ER), and mitochondrial Ca2+ has contributed immensely to our understanding of cellular physiology. Here we describe the measurement of peroxisomal Ca2+ using ratiometric Ca2+ sensors, enabling measurement of absolute Ca2+ concentration and its dynamics in living cells.
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