Live birth after transfer of vitrified-warmed embryo derived from vitrified-warmed oocyte and frozen-thawed sperm following failed ICSI: Case report.

Zhihong Zhang,Jihong Zhu,Hongyang Zhang,Dongfeng Geng,Qi Xi,Shu Deng,Ruizhi Liu

doi:10.4067/s0034-98872017000300017

Abstract

Since the first successful pregnancy from a frozen human oocyte was reported, remarkable technological progress has been made in the area of cryopreservation of human oocytes. We report a successful delivery of two healthy babies after transfer of vitrified-warmed embryos derived from intracytoplasmic sperm injection (ICSI) with vitrified-warmed oocytes and frozen-thawed sperm. A female patient and her husband with severe oligoasthenspermia are reported. At the day of oocyte collection, very few inactive sperms were found in her husband semen. Multiple site open testicular biopsy was performed on her husband, but no sperm was retrieved. The patient did not become pregnant after transferring two embryos coming from half of oocytes and inactive sperms. The patient got pregnant and delivered two healthy babies after receiving a transfer of vitrified-warmed embryos from vitrified-warmed oocytes and frozen-thawed sperm.

Highlights

We report a successful delivery of two healthy babies after transfer of vitrified-warmed embryos derived from intracytoplasmic sperm injection (ICSI) with vitrified-warmed oocytes and frozen-thawed sperm
We report the healthy outcome of two girls who were born after transfer of two vitrified-warmed embryos produced by ICSI using vitrified-warmed oocytes and frozen-thawed donor sperms
Cryopreservation of human oocytes has long been postulated to be a potentially useful addition to the range of techniques available in assisted reproduction laboratories. It would permit the storage of reproductive potential for women at risk of loss of fertility when undergoing cytotoxic therapy, allow storage of oocytes when sperm retrieval is not possible in a cycle of in vitro fertilization (IVF) treatment

Summary

Introduction

We report a successful delivery of two healthy babies after transfer of vitrified-warmed embryos derived from intracytoplasmic sperm injection (ICSI) with vitrified-warmed oocytes and frozen-thawed sperm. The patient did not become pregnant after transferring two embryos coming from half of oocytes and inactive sperms. The patient got pregnant and delivered two healthy babies after receiving a transfer of vitrified-warmed embryos from vitrified-warmed oocytes and frozen-thawed sperm.

Results

Conclusion