Liuweidihuang Pill Alleviates Inflammation of the Testis via AMPK/SIRT1/NF-κB Pathway in Aging Rats.

Huiming Ma,Na Hu,Qian Zou,Yuxin Wang,Dongmei Chen,Zhangjie Yang,Lingling Yang,Shuai Zhao,Ruiqin Cui

doi:10.1155/2020/2792738

Abstract

Liuweidihuang Pill (LP) is a traditional Chinese herbal formula that is often used in clinical practice to treat kidney deficiency syndrome. The present study investigated the antiaging effects of LP in a D-galactose- (D-Gal-) induced subacute aging rat model. The study also attempted to explore whether anti-inflammatory mechanisms that underpin the antiaging effects are mediated by the AMPK/SIRT1/NF-κB signaling pathway. Rats were subcutaneously injected with D-Gal at a dosage of 100 mg/kg/d for 8 weeks. Upon successful induction of aging in the rats, the animal was administered LP at 0.9 g/kg/d by gavage for 4 weeks. Proteins of the testis were subsequently examined by western blot analysis, and associated locations in the testicular tissue were determined by immunohistochemistry. We observed that LP exerted antiaging effects in aging rats following the activation of AMPK/SIRT1. It was also observed that LP inhibited the expression of NF-κB, thereby further attenuating inflammation of the testis. Therefore, LP can alleviate inflammation of the testis via the AMPK/SIRT1/NF-κB pathway in aging rats.

Highlights

Yuxin Wang,1 Zhangjie Yang,1 Lingling Yang,1 Qian Zou,1 Shuai Zhao,1 Na Hu,1 Dongmei Chen,2 Ruiqin Cui,3 and Huiming Ma 1,4
Studies have shown that senescence can result in proinflammatory phenotypes, including elevated levels of typical inflammatory markers such as interleukin-1 (IL-1) [7], IL-6, and tumor necrosis factor (TNF-α); increases in the levels of these markers can indicate senescence [8, 9]. e processes of aging and inflammation are closely related. e aging process is often accompanied by an imbalance of inflammatory homeostasis, which can lead to aging. us, some researchers have suggested that chronic inflammation can be used as a biomarker for aging [10]
MO, USA). e antibody to p-AMPK was purchased from Cell Signaling Technology (Boston, USA), and the antibody to silent information regulator of transcription 1 (SIRT1) was ordered from Affinity Biosciences (USA). e antibody to NF-κB (P65) was bought from BOSTER (Wuhan, China). e antibodies to AMPK, HO-1, IL-1, IL-6, and IL-10 were all purchased from Boiss (Beijing, China), and the antibodies to P21Waf1/Cip1, P16INK4A, and TNF-α were procured from Wanleibio (Shenyang, China)

Summary

Introduction

Yuxin Wang ,1 Zhangjie Yang ,1 Lingling Yang ,1 Qian Zou ,1 Shuai Zhao, Na Hu ,1 Dongmei Chen ,2 Ruiqin Cui ,3 and Huiming Ma 1,4. E present study investigated the antiaging effects of LP in a D-galactose- (D-Gal-) induced subacute aging rat model. E study attempted to explore whether anti-inflammatory mechanisms that underpin the antiaging effects are mediated by the AMPK/SIRT1/NF-κB signaling pathway. We observed that LP exerted antiaging effects in aging rats following the activation of AMPK/SIRT1. Erefore, LP can alleviate inflammation of the testis via the AMPK/SIRT1/NF-κB pathway in aging rats. It has previously been shown that the inflammatory response plays an important role in regulating aging [11]. Studies have shown that in aging testicles, the levels of the agingrelated indicators P19, P21, and P53 increase. Evidence-Based Complementary and Alternative Medicine the levels of superoxidase dismutase (SOD) activity and inflammatory cytokines TNF-α, IL-1, and IL-6 are significantly increased in aging testicles [13]

Methods

Results

Discussion

Conclusion