"Little Adjustment of Lowry and Biuret Methods to Get Better Absorbance of Proteins"

Yohannis Wondwosen

doi:10.26717/bjstr.2021.39.006367

Abstract

Yohannis Wondwosen1*, Sisay Addisu1, Tsehayneh Kelemu1, Maria Degef1 and Gashaw Tadele2 Author Affiliations 1Department of Medical Biochemistry, College of Health Sciences, Addis Ababa University, Addis Ababa, Ethiopia 2Department of Chemistry, College of Natural and Computational Sciences, Mizan Tepi University, Ethiopia Received: October 27, 2021 | Published: November 10, 2021 Corresponding author: Yohannis Wondwosen, Department of Medical Biochemistry, College of Health Sciences, Addis Ababa University, Addis Ababa, Ethiopia DOI: 10.26717/BJSTR.2021.39.006367

Highlights

Proteins are the most abundant macromolecules in a biological system and their accurate quantification is an important part of any biochemical investigation in life sciences [1]
Reagents and chemicals were obtained from the following sources: Egg albumin, Bovine Serum Albumin(BSA), FC reagent, Sodium carbonate, Gelatin powder, Potassium sodium tartrate, Casein, Cobaltous acetate, Cadmium sulfate, Sodium di chromate and Deoxycholate were obtained from British Drug House(BDH) British; Tri Carboxylic Acid(TCA) was obtained from May and Baker Company, England; Sodium Hydroxide was obtained from Fisher Scientific, United Kingdom; FC reagent was obtained from MERCK company, Germany; Tris Hydrochloride and Cupric Acetate were obtained from Labort Finch Chemical, India
Penta hydrated Cupric Sulfate was obtained from Osaka Hayashi pure Chemical Industries, Japan; Hemi Penta hydrated Copper nitrate, Sodium Sulfite, Sodium Dodecyl Sulfate (SDS) and Tannic acid were obtained from Sigma Aldrich, Chemicals, St

Summary

Introduction

Proteins are the most abundant macromolecules in a biological system and their accurate quantification is an important part of any biochemical investigation in life sciences [1]. Many colorimetric dye-based methods are avilable for the measurment of protein concentration in solutions [2,3,4] Out of those the most popular methods include Lowry [5,6,7], Biuret [8], Bradford [9] and Bicinconic acid [10]. Each of these methods has its own pros and cons with respect to sensitivity, reproducibility, rapidity and level of interference by non-protinaceous substances. Copper (II) Sulfate Penta Hydrate Cu SO4. 5H2O 248.68g/mol Crystalline solid 110 ̊C 2.284g/cm Best 248.93415 248.93 Deep blue

Materials and Methods

Results and Discussion

Conclusion