Litter sex composition influences competitive performance during first reproduction in male mouse lemurs

Abstract

In litter-bearing mammals, conditions experienced early in life can have long-lasting consequences on adult behavioral and physiological phenotypes, including reproductive fitness and survival. Using data from a large database, we focused our analysis on the consequences of litter composition on the reproductive performance of 131 mouse lemur males during their first breeding season. For male offspring, body mass at birth and at weaning only depended on the litter size (from one to 3), with the lowest values in triplets. Early growth had no relationship with the future reproductive success when males entered their first breeding season. When mouse lemurs were kept in groups with 2 or 3 competitors, males entered sexual competition for priority access to females in estrus, leading to a hierarchy with the dominant male ensuring the successful mating of the females. Genetic paternity tests showed that males born in same-sex litters (M, MM, MMM) were more competitive and fathered more offspring than males born in mixed-sex litters (MF, MMF, MFF), indicating the negative impact of a sister on male reproductive success. However, testosterone levels were unrelated to early growth or litter sex composition but were dependent on social interactions during sexual competition, with higher values in successful males. The effects of litter composition on the mating success of male mouse lemurs might mainly occur through social interactions between male offspring born in same-sex litters. Play fighting between juvenile males could play a major role in their acquisition of the skills required to succeed in sexual competition.