Abstract
Inspired by the aligned extracellular matrix and bioceramics in human bone tissue, we investigated the relative contributions of nanotopography and equine bone powders (EBPs) with human dental pulp stem cells (DPSCs) to the osteogenesis. Both nanotopography and EBPs independently promoted the osteogenesis of DPSCs, osteogenesis was further promoted by the two factors in combination, indicating the importance of synergistic design factor of guided bone regeneration (GBR) membrane. The osteogenesis of DPSCs was affected by the polycaprolactone-based nanotopography of parallel nanogrooves as well as EBPs coating. Interestingly, both nanopattern and EBPs affected the DPSCs morphologies; nanopattern led to cell elongation and EBPs led to cell spreading and clustering. Analysis of the DPSCs-substrate interaction, DPSCs-EBPs interaction suggests that the combined environment of both factors play a crucial role in mediating osteogenic phenotype. This simple method to achieve a suitable environment for osteogenesis via nanotopography and EBPs coating modulation may be regarded as a promising technique for GBR/GTR membranes, which widely used dental and maxillofacial surgery applications.
Highlights
Biomaterials-based scaffolds have garnered interest in medical fields, such as cell transplantation, tissue engineering, regenerative medicine, and drug delivery [1,2]
We examined the role of mechanically patterned substrate and equine bone powders (EBPs) using human-derived dental pulp stem cells (DPSCs) by analyzing morphological features and osteogenesis
We proposed the simple mothed for the fabrication of the guided bone regeneration (GBR)/GTR membrane combined the nanotopographic surface and EBPs coating to enhance the osteogenic behavior of
Summary
Biomaterials-based scaffolds have garnered interest in medical fields, such as cell transplantation, tissue engineering, regenerative medicine, and drug delivery [1,2]. Defined scaffolds (e.g., highly aligned nanomatrix) have been proposed based on previous studies indicating that living cells are highly sensitive to the local architecture of the complex and well-defined structures of the extracellular matrix (ECM) [3,4]. It means that the architecture of synthetic extracellular matrices (ECMs) inspired by target tissue for programmed stem cell behavior and response, such as proliferation and differentiation could be essential design factors of tissue-engineered scaffolds.
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