Abstract
acid cleavage. There, Cas13 was found to have significantly higher nucleic acid detection ability at a relatively faster speed. However, Cas13's detection duration was 60 minutes at a femtomolar level, but this is not sufficient for a point-of-care diagnostic. To tackle this, costeffective Loop-mediated isothermal amplification (LAMP) was used to amplify nucleic acid with high sensitivity in the attomolar level in under 20 minutes. Despite the rapid, sensitive nucleic acid detection of LAMP, they have non-specific interaction resulting in non-template control (NTC) signal that needs to be addressed. This NTC signal is due to the dimerization of LAMP primer sets which might lead to high false-positive. To work around this, Cas13 was used as a secondary probe for specific target recognition. For Cas13 to avoid false-positive detections, the guide RNAs must not overlap with the primers. Short and highly overlapping LAMP primer raised amplicons -size ranged from 1-60 nt -to avoid...
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