Abstract
Milk samples collected from 2060 dairy cows were examined for the presence of Listeria species. Following enrichment and plating on selective agar, confirmation of the isolates was based on biochemical tests followed by Christie, Atkins, Munch Petersen (CAMP) test, phosphatidylinositol-specific phospholipase C (PI-PLC) assay. The isolates were subjected to PCR assay for the hlyAgene. Listeria spp. were isolated from 139 (6.75%) samples; 105 (5.1%) were positive for L. monocytogenes; 2 (0.1%) for L. seeligeri, 18 (0.9%) for L. innocua and 2 (0.1%) for L. welshimeri. All 105 L. monocytogenes isolates were haemolytic and CAMP positive, however, 90 isolates were found to be positive by the PI-PLC assay and for the hlyA gene. Considering the ubiquitous distribution of L. monocytogenes, it is important to maintain strict hygiene and practice pasteurization of raw milk in order to minimize the risks of human infection.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
