Abstract
ABSTRACT: The aim of this study was to investigate Listeria monocytogenes in ham sliced in supermarkets in Recife city, Pernambuco state. In total, 40 samples of sliced ham were collected, and 25 g of ham was added to 225 mL of Demi Fraser broth. After incubation, 0.1 mL was inoculated in Fraser broth and, subsequently, sown in supplemented Listeria Selective Agar, based on Otaviani and Agosti. The following tests were carried out for confirmation purposes: Gram stain, motility test, catalase test and cAMP test. There was L. monocytogenes in 25% (10/40) of the samples. The presence of L. monocytogenes in ready-to-eat food, such as sliced ham, is likely related to lack of proper equipment-cleaning in supermarkets, a fact that poses great risk to public health.
Highlights
The aim of this study was to investigate Listeria monocytogenes in ham sliced in supermarkets in Recife city, Pernambuco state
The pathogen Listeria monocytogenes is the cause of listeriosis cases, and it stands out among agents responsible for such contaminations
The ubiquitous microorganism Listeria monocytogenes is found in different environments such as soil, water, animals and humans, a fact that hinders its elimination from foodprocessing environments (FIGUEIREDO, 2015; IACUMIN et al, 2016; LEONG et al, 2017)
Summary
The aim of this study was to investigate Listeria monocytogenes in ham sliced in supermarkets in Recife city, Pernambuco state. The pathogen Listeria monocytogenes is the cause of listeriosis cases, and it stands out among agents responsible for such contaminations This disease has low incidence, it accounts for high mortality rates (LIU et al, 2015; LUO et al, 2017); its manifestation can be invasive such as meningitis, septicemia and miscarriage, as well as non-invasive, like gastroenteritis followed by fever and vomiting (IACUMIN et al, 2016). It is essential to prevent cross contamination in processing facilities, mainly the contamination of chilled products, since this pathogen is a psychotropic microorganism This storage system does not demand any other post-processing stage to inactivate this microorganism (IACUMIN et al, 2016; LEONG et al, 2017); the aim of this study is to assess the presence of Listeria monocytogenes in ham sliced in supermarkets in Recife city, Pernambuco State
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