LIS1, an RNA-binding protein, regulates the physical properties of embryonic stem cells

Abstract

LIS1 (Lissencephaly-1) was identified as the first gene involved in a neuronal migration disorder. The dosage of the LIS1 protein is important for proper brain development, whereas the absence of the protein results in early embryonic lethality. LIS1 interacts with the molecular motor cytoplasmic dynein, and this interaction impacts multiple activities. In this study, we set out to investigate the functions of LIS1 in embryonic stem cells. We found that LIS1 interacts with numerous RNA-binding proteins, including the Argonaute complex, and that LIS1 binds directly to RNA.