Abstract
A practical and reliable method of differentiating T. rubrum and T. mentagrophytes, var. interdigitale, is described and compared with the methods of Edgecombe (5) (dextrose-potato-agar medium) and of Bocobo and Benham (4) (dextrose-corn flour-agar medium). The method is based on differences of specific density between T. rubrum and T. mentagrophytes. After incubation of 2 to 3 days in a test tube containing a 4% dextrose and 1% pepton solution (liquid Sabouraud's solution), 92.5% (211) of the 228 T. rubrum strains examined grew at the bottom of the tube while 96.5% (56) of 58 T. mentagrophytes strains developed at the surface of the solution. This represents a modification of the original method described by Götz (2) who utilized a 10% pepton water solution as selective medium. Our method has been found to be of a higher degree of reliability than that carried out by Götz and yet easy to perform and obtaining the results within 2–3 days.
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