Abstract
The purification of α-amylase from a crude enzyme preparation was carried out be liquid-liquid reversed micellar extraction. After a full forward and backward extraction cycle, the α-amylase was purified about 1·5-fold with 85% recovery. Most of the contaminating neutral protease in the crude enzyme preparation could be removed at the end of an extraction cycle. The separation factor of α-amylase to neutral protease reached about 10.
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