Abstract
Ischemia modified albumin detection has great significance in early diagnosis and treatment of myocardial ischemia. In this study, anti-IMA was immobilized on the surface of a glass slide using self-assembled DMOAP and PEI, the binding of antibody and antigen lead to topology-geomorphologic changes on the slide surface, which induced 5CB molecules orientation transformation and modulated light transmittance. Results showed that we can detect concentrations as low as 50 μg/mL IMA. Moreover, this method can be realized with label-free, easy operation without dependence on equipment, and can be observed by the naked eye.
Highlights
Acute coronary syndrome (ACS) is a common disease in the cardiovascular system with extremely high mortality and morbidity, so early diagnosis of ACS is important to improve patients’ prognosis and quality of life [1]
dimethyl-N-octadecyl (3-aminopropyl) trimethoxysilyl chloride (DMOAP) was fixed on the upper slide, and mixed self-assembled DMOAP/PEI was immobilized on the bottom slide, which was prepared for binding anti-ischemia modified albumin (IMA)
When a liquid crystal (LC) cell was inserted with 5CB molecules and placed in a polarized microscope, LC molecules were uniformly placed with a vertical orientation that light could not filter through the cell, and the optical signal displayed as a uniform black image
Summary
Acute coronary syndrome (ACS) is a common disease in the cardiovascular system with extremely high mortality and morbidity, so early diagnosis of ACS is important to improve patients’ prognosis and quality of life [1]. Necrotic biochemical markers, such as creatine kinase isoenzyme MB (CK-MB), myoglobin (Myo), and cardiac troponin (cTn), are regarded as a useful tool for clinical treatment [3], these makers are only released from myocardial cells after irreversible lesions. CTn can be detectable in patients about 6 h after heart disease occurs. When they have ischemia symptoms, the level of cTn is under clinical decision limits such that patients have to stay in hospital for repeated tests [4]. Seeking for a reliable biochemical marker of myocardial ischemia appears important before cTn is generated
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