Abstract
The Liquid Chromatography-Mass Spectrometry (LC-MS) is a powerful analytical technique with very high sensitivity and specificity. LC-MS is combination of Liquid Chromatography (LC) and Mass Spectrometry (MS). With the Liquid Chromatography (LC) the separation of components can be done and then the sample eluents from LC are transferred into Mass Spectrometry (MS) where the detection, identification and determination of masses of components can be done in presence of other components. LC-MS is used in determination, of pharmaceutical drug substances, intermediates and its related compounds for quantitative and qualitative purpose. LC-MS is used most significantly in in-vitro dissolution, bio-equivalence, bioavailability and metabolite studies. Also LC-MS is used in basic research, agrochemical, forensic laboratories and food industries .In this article principle of LC-MS, instrumentation and its applications are briefly discussed.
Highlights
Liquid Chromatography-Mass Spectrometry (LC-MS)The High Performance Liquid chromatography (HPLC) is one of most common analytical technique used in pharmaceutical industry for determination and quantification of drug substances and its related substances
Preparative LC-MS systems can be used for rapid mass-directed purification of specific substances from such mixtures that are important in basic research, pharmaceutical, agrochemical, food and other industries [2,3]
The Liquid Chromatography-Mass Spectrometry (LC-MS) is combination of Liquid Chromatography and Mass Spectrometry which is used with separation power of HPLC with detection power of Mass Spectrometry (MS)
Summary
The High Performance Liquid chromatography (HPLC) is one of most common analytical technique used in pharmaceutical industry for determination and quantification of drug substances and its related substances. HPLC (LC) separates the components of mixtures by passing through chromatographic column. Mass spectrometry is alone not good for identifying mixtures because mass spectrum mixture is complex of overlapping spectra from separated individual components. It is difficult to connect Liquid chromatography (LC) with Mass spectrometry (MS). The Liquid Chromatography-Mass Spectrometry (LC-MS) is combination of Liquid Chromatography and Mass Spectrometry which is used with separation power of HPLC with detection power of Mass Spectrometry (MS). Liquid Chromatography (HPLC): The Liquid Chromatography (LC) is a high performance liquid chromatography in which separation of components of mixture can be carried out by using liquid mobile and solid stationary phase. Liquid Chromatography-Mass Spectrometry and Its Applications: A Brief Review.
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