Abstract
A new liquid chromatography–tandem mass spectrometry (LC-MS/MS) method is developed for the quantification of dehydrodiisoeugenol (DDIE) in rat cerebral nuclei after single intravenous administration. DDIE and daidzein (internal standard) were separated on a Diamonsil™ ODS C18 column with methanol–water containing 0.1% formic acid (81:19, v/v) as a mobile phase. Detection of DDIE was performed on a positive electrospray ionization source using a triple quadrupole mass spectrometer. DDIE and daidzein were monitored at m/z 327.2→188.0 and m/z 255.0→199.2, respectively, in multiple reaction monitoring mode. This method enabled quantification of DDIE in various brain areas, including, cortex, hippocampus, striatum, hypothalamus, cerebellum and brainstem, with high specificity, precision, accuracy, and recovery. The data herein demonstrate that our new LC-MS/MS method is highly sensitive and suitable for monitoring cerebral nuclei distribution of DDIE.
Highlights
IntroductionThe seed of Myristica fragrans Houtt (family: Myristicaceae) is broadly utilized as a spice and flavoring in African, Asian, and Western sustenance
Nutmeg, the seed of Myristica fragrans Houtt is broadly utilized as a spice and flavoring in African, Asian, and Western sustenance
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Summary
The seed of Myristica fragrans Houtt (family: Myristicaceae) is broadly utilized as a spice and flavoring in African, Asian, and Western sustenance. A few studies have uncovered that nutmeg extract has the capacity to stimulate the nervous system [2,3,4] and diminish intestinal tumorigenesis in Apcmin/+ mice [5]. Dehydrodiisoeugenol (DDIE) is one of the major neolignanoids found in nutmeg [6,7] and the aril [8] of M. fragrans. DDIE exhibites different bioactivities in vitro and in vivo, including: antibiotic [8], anti-inflammatory [9], anti-tumor [10], and anti-oxidation [11,12,13]. It was found that DDIE extraordinarily restrained the expression of iNOS mRNA [16] and can promptly cross the blood-brain
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