Abstract
A high-performance liquid chromatographic-thermospray mass spectrometric method was developed for analysis of toremifene and eight of its derivatives, including some main metabolites. The compounds were chromatographed with an acetonitrile-ammonium acetate buffer eluent. Thermospray mass spectra of the compounds, showing abundant protonatede molecular ions, were obtained with the exception of deaminohydroxy- and deaminocarboxy-toremifene, which apparently did not get protonated under these conditions. Selected-ion monitoring (SIM) of the protonated molecular ion of toremifene, the molecule of main interest in this series, was accomplished. According to the results, toremifene can be analyzed in the range from 0.01 μg/ml to 10 μg/ml, the limit of detection being 500 pg per injection. This method provides an effective means for purity assignation of forthcoming synthetic triphenylethylene derivatives. Liquid chromatography-thermospray mass spectrometry with selected-ion monitoring seems to be a sensitive and a selective quantitation method for toremifene.
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