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Abstract
A liquid chromatographic method for analysis of tylosin A and related substances was developed using wide-pore poly(styrene-divinylbenzene) as the stationary phase. As a mobile phase, a mixture of tetrahydrofuran-0.2 M potassium phosphate buffer pH 9.0-water (20:5:75) was used at a flow-rate of 1.0 ml/min. The column was kept at 60°C. Detection was performed by UV at 280 nm. Tylosin A is separated from all its known related substances that are potentially present as impurities in commercial samples of tylosin base and tylosin tartrate. The method shows good linearity and repeatability and is stability indicating. Results for a number of commercial samples analysed using the newly developed method are presented.
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