Abstract
We have developed a fluorescence derivatization method of 5-hydroxyindoles (serotonin, 5-hydroxyindole-3-acetic acid, N -acetylserotonin) based on benzylamine derivatization, followed by photocatalytic oxidation. In the present study, a photocatalyst, which was titanium dioxide supported on a diatomaceous earth particle, was used instead of potassium hexacyanoferrate(III) as a chemical oxidizing agent employed in the conventional method. The resulting fluorescent derivatives were separated by reversed-phase liquid chromatography on an ODS column by using a mixture of acetonitrile and 250 mM acetate buffer (pH 4.6) and detected spectrofluorimetrically at 465 nm with excitation at 350 nm. The detection limit (signal-to-noise ratio = 3) for N -acetylserotonin was 16 fmol per 20 μl injection.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
