Abstract
A rapid, selective, and sensitive LC-MS/MS method has been developed and validated for quantification of lansoprazole (LZ) in human plasma using esomeprazole as an internal standard (IS). The analyte and IS were extracted by single solvent liquid-liquid extraction using tert-Butyl methyl ether and separated by isocratic elution on C18 analytical column with 90:10 v/v acetonitrile, and 20 mM ammonium acetate (flow rate of 1 mL/min) as the mobile phase in the positive ion mode. Selected Reaction Monitoring transitions for LZ, internal standard and their daughter ion were observed at 369, 346, 252, and 198 m/z, respectively. The lower limit of quantification was 3.997 ng/mL and total run time for the analysis was 1.2 minutes. Linear calibration was observed in the range of 3.997 ng/mL to 2002.5 ng/mL. The method was validated for its selectivity, stability, accuracy, precision, and recovery.
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