Liquid chromatography — electrochemical detector for the determination of glucose in rat brain combined with in vivo microdialysis

Abstract

In this paper, microdialysis sampling was combined with liquid chromatography with electrochemical detection (LC-ECD) and glucose in rat brain was determined. A cobalt tetraaminophthalocyanine (CoTAPC)/glucose oxidase (GOD)/Nafion film-coated glassy carbon electrode was used to selectively detect the hydrogen peroxide generated enzymatically in the enzyme reactors, without any interference from electroactive species such as l-ascorbate, urate, proteins. The signal current was linearly related to the glucose concentration in the range of 1.0×10 −6–5.0×10 −3 mol/l at +0.5 V (versus Ag/AgCl) with a detection limit of 5.0×10 −7 mol/l. To measure glucose in rat brain, microdialysis sampling was employed. The dialysate from the microdialysis system was injected into system. The result was 0.74±0.10 mmol/l ( n=4).