Abstract
This chapter summarizes the possibilities of coupling of chromatographic separation techniques and mass spectrometry in the lipidomic analysis. We focus on liquid chromatography and supercritical fluid chromatography separation approaches because they offer the best potential for the analysis of various classes of lipids. Chromatographic approaches in lipidomic analysis are classified into three groups: lipid class separation, lipid species separation, and other separation approaches for dedicated applications, such as silver ion chromatography, chiral chromatography, and multidimensional configurations. The lipid class separation is represented by normal-phase, hydrophilic interaction chromatography, and ultrahigh-performance supercritical fluid chromatography, while reversed-phase chromatography is a typical mode used for the lipid species separation strategy. The advantages and limitations of individual approaches are discussed.
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