Liquid Chromatograph-Mass Spectrometry-Based Non-targeted Metabolomics Discovery of Potential Endogenous Biomarkers Associated With Prostatitis Rats to Reveal the Effects of Magnoflorine.

Yin Yuan,Hong-Bin Xiao,Xu Liu,Fei-Xue Dong,Zhong-Guang Zhou

doi:10.3389/fphar.2021.741378

Abstract

Magnoflorine (Mag) has multiple pharmacological activities for the prevention and treatment of prostatitis. However, its molecular mechanisms andpharmacological targets are not clear. In this study, the ultra-performance liquid tandem mass spectrometry-based metabolomics method was used to clarify the intervention of Mag against prostatitis and the biological mechanism. A total of 25 biomarkers associated with the prostatitis model were identified by metabolomics, and a number of metabolic pathways closely related to the model were obtained by MetPA analysis. After given Mag treatment, the results of each indicator were shown that Mag alkaloid could inhibit the development of prostatitis effectively. We found that Mag had regulative effects on potential biomarkers of prostatitis model, which can regulate them to the control group. Our results indicated that alkaloids have an effective intervention therapy for prostatitis, and five types of metabolic pathways closely related to prostatitis model were obtained, including phenylalanine, tyrosine and tryptophan biosynthesis, phenylalanine metabolism, tyrosine metabolism, arginine and proline metabolism, glycine, serine and threonine metabolism, alanine, aspartate and glutamate metabolism. This study has provided the basic experimental data for the development of Mag in the prevention and treatment of prostatitis.

Highlights

Metabolomics focuses on the comprehensive analysis of overall metabolites in biological samples, including known and unknown metabolites (Zhang et al, 2012; Liang et al, 2015; Li et al, 2018), and give more emphasis on the identification of unknown metabolites
The results showed that the protein exudation in the model group was obviously higher than the control group (p < 0.01)
The results of eye movement and activity, protein exudation and histopathological sections showed that the pain symptoms of prostatitis occurred after capsaicin injection into the prostatic tissue of the rats, which indicated that the rat model of prostatitis was replicated successfully

Summary

Introduction

Metabolomics focuses on the comprehensive analysis of overall metabolites in biological samples, including known and unknown metabolites (Zhang et al, 2012; Liang et al, 2015; Li et al, 2018), and give more emphasis on the identification of unknown metabolites. Metabolomics is often analyzed by mass spectrometry (MS) technology in the mode of multiple reaction monitoring (MRM) and parallel reaction monitor (Zhang et al, 2013a; Liang et al, 2016a; Nan et al, 2016). Metabolite Biomarkers Reveal Magnoflorine Effects measuring metabolites with robustness and high-throughput properties (Zhang et al, 2015; Wang et al, 2019; Pareek et al, 2020). Liquid chromatography-mass spectrometry (LC-MS) is the most widely used metabolomics analysis platform because of its high resolution and sensitivity (Zhang et al, 2013b; Liang et al, 2016b; Zhang et al, 2018). Metabolomics is a powerful approach to discovering the disease biomarkers, metabolic mechanisms and targets for drugs (Li et al, 2017; Xiong et al, 2020; Xiong et al, 2021)

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Discussion

Conclusion