Abstract
Abstract A high-pressure liquid chromatographic procedure has been developed for the separation of 6 major alkaloids in gum or powdered opium. The alkaloids are absorbed from an aqueous dimethylsulfoxide solution onto a strong cation exchange resin and eluted with methanolammonia; the solvent is removed by reduced pressure evaporation. The alkaloids from the residue are then dissolved in chloroform-methanol (3+1). The sample solution, plus brucine alkaloid as an internal standard, is placed on a Corasil Type II column (microbore) and eluted with hexane that is gradient programmed with a solution of chloroform-methanol-diethylamine (100+300+1). The absorbance of the eluate is continuously monitored in a flow-through ultraviolet photometer.
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