Liquid Chromatographic Separations Using Rubber as the Stationary Phase

Abstract

Abstract In a previous communication it was reported that certain polymers could be separated from mineral oil by a liquid chromatographic method. n-Heptane was used as the mobile phase and small pieces of thin vulcanized natural latex as the stationary phase. It has now been found that compounds of lower molecular weight can also be separated by this method. The use of rubber for the separation of compounds of low molecular weight was first described by Boldingh; he found it advantageous first to swell the rubber with a different solvent from that used as the mobile phase. The use of a crosslinked dextran (“Sephadex”) for separating compounds in aqueous solution has been described by Gelotte. Vaughan has mentioned the possibility of using crosslinked polystyrene beads for this purpose.