Liquid Chromatographic Separationa of Calixarenes

Abstract

In this paper. the retention behavior of p-t-butyl calixarenes: p-t-butylcalix[4]arene, p-t-butylcalix[5]arene, p-t-butylcalix[6]-arene, p-t-butylcalix[7]arene, p-t-butylcalix[8]arene, p-t-butylcalix[9]arene, p-t-butylcalix[10]arene and the bis-homooxacalix[4]arene was examined by reverse phase liquid chromatography, on a silica based octadecyl bonded phase with non-aqueous tertiary eluent in a linear gradient elution. The effect of various mobile phase compositions on the capacity ratio, peak asymmetry and resolution were studied. It was found that log k' did not vary linearly with the percentage of methanol, and that the symmetry of peaks was improved when shorter elution times and/or methanol percentage were decreased.