Abstract
A simple and sensitive RP-HPLC method was developed and validated for the determination of Candesartan cilexetil and Hydrochlorthiazide in pharmaceutical dosage forms. The separation of components was achieved on a SHIMADZU Hypersil ODS-C 18 column (250 × 4.6 mm, 5 µm) with UV detection at 270 nm. Isocratic elution with a mobile phase consisting of 10 mM (pH 3.37) Tetra butyl ammonium hydrogen sulphate: methanol (15:85, V/V), at a flow rate 1.0 mL min -1 was employed. Linearity was observed in the concentration range 0.625-62.5 µg/mL for Hydrochlorthiazide and 0.8-80 µg/mL for Candesartan cilexetil respectively. The linear regression equation was found to be Y=64002X-1412.6 for Hydrochlorthiazide and Y=24649X-6701.8 for Candesartan cilexetil respectively with correlation coefficients greater than 0.999. The LOD was found to be 0.1385 and 0.1892 µg/mL for Hydrochlorthiazide and Candesartan cilexetil respectively where as the LOQ was found to be 0.4394 and 0.6187 µg/mL for Hydrochlorthiazide and Candesartan cilexetil respectively. The mean analytical recovery in determination of Candesartan cilexetil and Hydrochlorthiazide tablets was 99.31-100.08% Hydrochlorthiazide and 99.58-100.39% for Candesartan cilexetil respectively. Thus, the proposed method is applicable for routine determination of Candesartan cilexetil and Hydrochlorthiazide in pharmaceutical formulations.
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