Abstract
A liquid chromatographic method is described for the analysis of all-rac-α-tocopheryl acetate and retinyl palmitate in soy based infant formula. The vitamins are extracted from infant formula without saponification by matrix solid phase dispersion (MSPD) and quantitated by normal phase chromatography with fluorescence detection. Retinyl palmitate and all-rac-α-tocopheryl acetate are quantitated isocratically with a mobile phase of hexane containing isopropanol @ 0.125% (v/v) and 0.5% (v/v), respectively. Results compared favorably to the label declaration on a retail infant formula product.
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