Abstract
Abstract An isocratic normal-phase liquid chromatographic (LC) method for measuring methazole in technical and formulated products was collaboratively studied by 12 laboratories. Samples of technical or formulated products are dissolved in 1,4-dioxane-hexane containing 2-chloro-5-nitrobenzophenone as internal standard. Following centrifugation and further dilution, the sample is analyzed by LC on a 25 cm x 4.6 mm stainless steel column packed with 5 μm silica, with ultraviolet detection at 254 nm. Premixed 1% 2-propanol in hexane is used as the mobile phase. Peak areas are obtained at the retention times corresponding to the internal standard and methazole. The quantity of active ingredient is determined by comparing the area ratio of active ingredient to internal standard in the sample with the same ratio in a single calibration solution. Collaborators were provided with 1 Youden matched pair for each of the technical, 75% wettable granular, and 4 lb/gal flowable products studied. The reproducibility relative standard deviations (RSDR) for the product types studied were 1.05% for technical, 1.80% for granular, and 4.76% for flowable. The method has been adopted first action by AOAC International.
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