Liquid Chromatographic Determination of Flavoxate HCl in Pharmaceutical Formulation

M Attimarad

doi:10.4103/0975-1483.66787

Abstract

The objective of the study was to develop a high performance liquid chromatography (HPLC) method using ultra violet (UV) detection for the determination of flavoxate HCl in bulk and solid dosage forms by using ibuprofen as the internal standard. Eclipse C18 column (150 mm × 4.6 mm, 5 μm) was used as the stationary phase with a mixture of acetonitrile : 0.1% formic acid in water (75: 25 v/v) as the mobile phase. The response of the drug was linear in the concentration range of 1 – 250 μg/ml. Limit of detection and Limit of quantification were found to be 0.23 μg/ml and 0.69 μg/ml, respectively. The percentage of recovery ranged between 97.4 and 101.3%. The factors affecting column separation of the analyte were studied. The results demonstrated that this method is reliable, reproducible, and suitable for routine quantitative use.

Highlights

Flavoxate hydrochloride (FLX, 3-methylflavone-8-carboxylic acid β-piperidinoethyl ester hydrochloride, C24H26ClNO4, MW : 427.93, Figure 1), belongs to a series of flavone derivatives, which exhibit strong smooth muscle relaxant activity, with selective action on the pelvic.[1]
The literature survey reveals that FLX was estimated from its pharmaceutical preparations by using Ultraviolet spectrophotometr y,[3,4] high perfor mance liquid chromatography (HPLC),[5,6,7,8,9] Voltammetry,[10] capillary electrophoresis,[11] and potentiometric[12] determination techniques
The retention time of the reported HPLC methods is more than five minutes and the flow rate of the mobile phase is 1.5 ml/min, which requires a greater mobile phase for the determination of flavoxate

Summary

Introduction

Flavoxate hydrochloride (FLX, 3-methylflavone-8-carboxylic acid β-piperidinoethyl ester hydrochloride, C24H26ClNO4, MW : 427.93, Figure 1), belongs to a series of flavone derivatives, which exhibit strong smooth muscle relaxant activity, with selective action on the pelvic.[1]. The retention time of the reported HPLC methods is more than five minutes and the flow rate of the mobile phase is 1.5 ml/min, which requires a greater mobile phase for the determination of flavoxate. In the present study it was intend to develop a rapid, economical, simple, and precise RP HPLC method for the estimation of flavoxate HCl in bulk and formulations.

Objectives

Results

Conclusion