Abstract
The use of high-performance liquid chromatography (HPLC) for the characterization of the adducts formed by the32P-postlabeling of DNA is described. Adducts formed from the reaction of DNA with small molecules are characterized as deoxyribonucleoside-5′-monophosphates. Adducts formed from the reaction of DNA with larger molecules are characterized as 3′,5′-bisphosphates. In either case, good separations are obtained for adducts, other than methyl, by conventional reversed-phase chromatography using a C18 column. Ion-exchange chromatography can be used in selected circumstances where reversed-phase techniques are not successful. Using sample concentration techniques, sensitivities are achievable which make this technique applicable toin vivo situations.
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