Abstract
Abstract A sensitive and selective HPLC assay has been developed for the analysis of a new antihypertensive agent in human plasma. The drug and internal standard were isolated from plasma by liquid-liquid extraction. Separation was accomplished on unmodified silica gel with a mobile phase of 60:40 acetonitrile:10 mM dibasic ammonium phosphate. Detection was by UV absorbance at 291 nm. The method is linear over a range of 20 to 4000 ng/ml. Relative error of calibration and control standards ranged from −1.5 to 5.0% and precision, as indicated by relative standard deviation, ranged from 0.8 to 5.2%. The method has been successfully used for analysis of plasma samples from human volunteers following oral administration of PD 78,799.
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