Abstract
The percentages of sperm motility and normal acrosome on the liquid boar semen diluted and preserved at 4°C with lactose hydrate, egg yolk and N-acetyl-D-glucosamine (LEN) diluent were significant differences according to preservation day and incubation time, respectively. The sperm motility steadily declined from 96.9% at 0.5 h incubation to 78.8% at 6 h incubation at 1 day of preservation. However, the sperm motility rapidly declined after 4 day of preservation during incubation. The normal acrosome steadily declined from 93.3% at 0.5 h incubation to 73.8% at 6 h incubation at 1 day of preservation. However, the normal acrosome rapidly declined after 3 day of preservation during incubation. The rates of sperm penetration and polyspermy were higher in 5 and 10×10 6 sperm/ml than in 0.2 and 1×10 6 sperm/ml. Mean numbers of sperm in penetrated oocyte were highest in 10×10 6 sperm/ml compared with other sperm concentrations. The rates of blastocysts from the cleaved oocytes (2-4 cell stage) were highest in 1×10 6 sperm/ml compared with other sperm concentrations. In conclusion, we found out that liquid boar sperm stored at 4°C could be used for in vitro fertilization of pig oocytes matured in vitro. Also, we recommend 1×10 6 sperm/ml concentration for in vitro fertilization of pig oocytes. (Asian-Aust. J. Anim. Sci. 2004. Vol 17, No. 10 : 1369-1373)
Highlights
The percentages of sperm motility and normal acrosome on the liquid boar semen diluted and preserved at 4°C with lactose hydrate, egg yolk and N-acetyl-D-glucosamine (LEN) diluent were significant differences according to preservation day and incubation time, respectively
There were significant differences on normal acrosome according to preservation day and incubation time
Mean numbers of sperm in penetrated oocyte were highest in 10×106 sperm/ml compared with other sperm concentrations
Summary
IVF of in vitro matured pig oocytes using liquid boar sperm stored at 4°C was not reported. In vitro evaluation of sperm quality is important for this study was carried out to evaluate liquid research on sperm preservation and in vitro fertilization boar sperm quality during storage, and investigate IVF and (IVF) of pig oocytes. Sellés et al (2003) reported culture of pig oocytes inseminated by liquid boar sperm that the IVF system was a good tool to evaluate the quality preserved at 4°C. of frozen-thawed boar semen previous to its commercial way and a good way to assay new sperm freezing
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
